Microdissection of Drosophila polytene chromosomes for DOP-PCR.
نویسنده
چکیده
This protocol describes a method for the microdissection of Drosophila polytene chromosomes. The microdissected DNA can be amplified by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) and then used to prepare probes for fluorescent in situ hybridization (FISH).
منابع مشابه
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ورودعنوان ژورنال:
- Cold Spring Harbor protocols
دوره 2012 3 شماره
صفحات -
تاریخ انتشار 2012